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Diagnostics

VetoSlide: A Diagnostic Tool For Clinical Mastitis

VetoSlide

VetoSlide is a dual slide culture for mild to moderate cases of mastitis.
It is a simple process; all it requires is a VetoSlide test kit and an incubator.
On diagnosing a case of mastitis, take a sterile milk sample from the affected quarter and inoculate each side of the plate by applying milk in a zig zag pattern using the cotton swab provided. The slides are then incubated for 24 hours.

Vetoslide Vetoquinol - Easily identify bacteria in milk samples within an 8-24 hour period
vetoslide

The Benefits of VetoSlide

  • Simple to use and interpret
  • Enables easy identification of Gram-positive and Gram-negative bacteria
  • Provides accurate results within 8-24 hours.
  • Allows informed and targeted mastitis treatment
    decisions on a case-by-case basis
  • Is conveniently stored at room temperature.

Why Use VetoSlide?

Mastitis caused by Gram-negative bacteria have a high self-cure rate and up to 30% of clinical mastitis samples do not grow on culture.

 

Evidence suggests, in mild to moderate cases, these cases infrequently benefit from antimicrobials.


In contrast, antibiotic treatment will significantly improve bacteriological cure rates when Gram-positive pathogens are responsible for the infection.

VetoSlide is reliable

VetoSlide has been shown to be accurate with an overall sensitivity and specificity of 82.0% and 76.8% respectively for Gram-positive pathogens and 83.3% and 94.3% respectively for Gram-negative pathogens. Looking exclusively at E.coli, the specificity increases further to 97.5%. This means very few Gram-negative growths will receive unnecessary antibiotic treatment.

Using vetoslide as a treatment decision tool can help reduce antimicrobial use

Utilising VetoSlide on mild to moderate clinical mastitis cases establishes if the infection is likely to be Gram-positive or Gram-negative in origin. This helps to distinguish those Gram-negative or no-growth animals that only require anti-inflammatory treatment and are unlikely to need antibiotics, from those infected with Gram-positive pathogens who are more likely to require antibiotic intervention. We always recommend immediate treatment for sick cows with severe clinical mastitis.

Interpreting VetoSlide

After 8-12 hours the slides can be checked to see if there are any colonies on the green media. Growth indicates the presence of Gram-negative bacteria. In addition, red colonies on the green media suggests Escherichia coli infection. Continue to incubate for the full 24 hours to confirm any Gram-positive colonies on the red side. Growth on both sides would imply a mixed infection or a contaminated sample.

To Find Out More About VetoSlide

Download the brochure to find out how you can use VetoSlide as a treatment decision tool that can help reduce antimicrobial use, along with examples of different colony growth.

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We are part of the essential milk industry, so we are part of the solution.

We provide you with the tools you need to give the best advice, take preventive action, and make evidence-based decisions.

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Mastitis Therapy

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